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KMID : 0861020020170020071
Korea Journal of Herbology
2002 Volume.17 No. 2 p.71 ~ p.83
Isolation and Identification of Soil Microorganisms in Wild Ginseng Radix of Moo-Ju Area





Abstract
objectives : Microorganisms were isolated from soils remaining attached to the roots of Wild Panax ginseng C.A. Mey. naturally grown in Moo-Ju area.
methods : As a preliminary study for the establishment of wild ginseng radix discrimination system based on area of growth, these isolates were identified by using mainly API 20NE and API 50CHB kits and the microbial consortia were compared. From the sample of Moo-Ju area, 9 and 6 strains were isolated and identified after the incubation of agar plates at 30¡É and 37¡É, respectively.
results : The results showed that 2 strains of soil bacteria, Aeromonas hydrophilia, and Bacillus cereus commonly existed in the roots of wild ginseng of both 30¡É and 37¡É. And that 6 strains, Pseudomonas putida, Stenotrophomonas maltophilia, Pseudomonas fluorescence at 30¡É and Bacillus subtilis, Empedobacter brevis, Brevi. laterosporus at 37¡É, inhabited specifically in the roots of wild ginseng of Moo-Ju area.
conclusion : These results suggest that comparison of microbial consortia associated with roots of wild ginseng could be used for the establishment of a naturally growing wild ginseng radix discrimination system.
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